Inhibitory effect of STAT3 antisense oligodeoxynucleotide against human adenocarcinoma of lung derived from A549 cell in nude mice
10.3724/SP.J.1008.2011.00076
- Author:
Ding SUN
1
Author Information
1. Department of Atomic Irradiation Prevention
- Publication Type:Journal Article
- Keywords:
Adenocarcinoma;
Antisense oligonucleotides;
Antitumor effect;
Lung neoplasms;
Neoplasm transplantation;
STAT 3
- From:
Academic Journal of Second Military Medical University
2011;32(1):76-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effects of antisense oligodeoxynucleotide(ASODN) of signal transducer and activator of transcription 3 (STAT 3) on development and growth of transplanted lung A549 cell adenocarcinoma in nude mice. Methods: The inhibitory effects of STAT 3 ASODN on A549 cell growth was evaluated by CCK-8 assay. STAT 3 ASODN-treated A549 cells were implanted subcutaneously into nude mice; the tumor growth was observed and the tumor-forming rate was calculated. After subcutaneous implantation of the untreated A549 cells, the tumor-bearing mice were randomized into 3 groups: control group, STAT 3 ASODN group, and nonsense oligodeoxynucleotide (NSODN) group, with normal saline, STAT 3 ASODN, and STAT 3 NSODN injected intatumorally (15 mg/kg daily for 3 weeks). The tumor volumes were measured every other day and the tumor weights were determined. The protein expressions of STAT 3 and p-STAT 3 in xenografts were examined by Western blotting analysis. Results: STAT 3 ASODN significantly inhibited the growth of A549 cells compared with STAT 3 NSODN group (P<0.05). The tumor formation ability of A549 cells treated with STAT 3 ASODN was significantly decreased (with the tumor inhibitory rate being 75.8%). Moreover, the growth of transplanted tumor was also significantly inhibited in STAT 3 ASODN group as compared with those in NSODN group and control group (P<0.05) . The tumor weights were significantly reduced in STAT 3 ASODN group than in STAT 3 NSODN group and control group (with the tumor inhibitory rate being 51.1%). The expression of STAT 3 protein and p-STAT 3 was also down-regulated in STAT 3 ASODN group. Conclusion: STAT 3 ASODN can suppress the tumorigenic ability of A549 cells and inhibit the growth of transplanted tumor in nude mice.
