Reprogramming of adipose-derived stem cells of hepatic cancer patients into pluripotent stem cells
10.3724/SP.J.1008.2011.00382
- Author:
Yu-Hua FU
1
Author Information
1. Xinyuan Institute of Medicine and Biotechnology
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells;
Induced pluripotent stem cells;
Liver neoplasms;
Reprogramming;
Retroviru
- From:
Academic Journal of Second Military Medical University
2011;32(4):382-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reprogram the adipose-derived stem cells of hepatic cancer patients into induced pluripotent stem cells (iPS cells). Methods Retrovirue-based pMXs containing Oct4, Sox2, Klf4 and c-Myc were prepared and were used to infect adipose-derived stem cells; the infected cells were then cultured. Alkaline phosphatase staining, quantitative-PCR and immunofluorescence assay were used to identify the ES-cell-like colonies. Results The ES-cell-like colonies expressed Alkaline phosphatase. Quantitative-PCR showed that ES-cell-like cells expressed the pluripotency genes of ES cells. Furthermore, immunofluorescence confirmed the expression of Oct4 and Sox2 in ES-cell-like cells. Conclusion Adipose-derived stem cells of hepatic cancer patients can be efficiently reprogrammed into iPS cells, and they can serve as the feeder layers of their own iPS cells. These findings may pave a way for improving efficiency of reprogramming adult cells and establishing hepatic cancer model for inducing pluripotent stem cells.
