Improvement in specimen preparation of ultra-thin section staining for transmission electron microscopy
10. 3724/SP. J. 1008. 2011. 00559
- Author:
Kai TANG
1
Author Information
1. Department of Physics
- Publication Type:Journal Article
- Keywords:
Specimen preparation;
Transmission electron microscopy;
Ultra-thin section's staining
- From:
Academic Journal of Second Military Medical University
2011;32(5):559-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To improve the staining efficiency and quality of biological specimens ultra-thin section for transmission electron microscopy. Methods: The "plug-in staining method" and continuous staining were developed by modifying the traditional staining method. Results: Using our method, we completed the electron staining of a large number of ultra-thin sections of biological specimens of within a short period of time. Conclusion: Compared with traditional method, the new method can save time and drug, and reduce the possibility of staining contamination. The ultrastructure observed by electron microscope is more clear and distinct.
