Lanthanum chloride induces apoptosis in HeLa cells via Caspase-3 pathway
10.3724/SP.J.1008.2011.00541
- Author:
Fei GUO
1
Author Information
1. Burns Institute
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Caspass-3;
HeLa cells;
Lanthanum
- From:
Academic Journal of Second Military Medical University
2011;32(5):541-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore apoptosis-inducing effect of lanthanum chloride in cervical cancer cells in vitro and the related mechanism, so as to provide a theoretical basis for medical use of rare earth complex. Methods: The anti-tumor activity of lanthanum chloride against HeLa cells was examined by morphological observation and MTT (3-[4, 5-dimethyrthiazol-2-yl] - 2, 5-diphenyltetrazolium bromide) assay. Distribution of cell cycle and cell apoptosis were analyzed using flow cytometry (FCM). The expression and activation of Caspass-3 were examined by Western blotting assay and spectrophotometry. Results: Morphological observation and MTT assay showed that lanthanum chloride significantly induced apoptosis in HeLa cells and inhibited cell growth in a doss-dependent manner (P<0.05). FCM analysis showed that the cell ratio of G0/G1 phase increased and that of S phase decreased significantly with the increase of lanthanum chloride doses. Lanthanum treatment also induced cell apoptosis doss-dependently. The minimum dose of lanthanum chloride to trigger apoptosis was 5 μmol/L, and the apoptosis rate increased in a doss-dependent manner compared with the control group (P<0.01). Western blotting analysis showed that lanthanum chloride up-regulated Caspass-3 expression in a doss-dependent manner. Conclusion: Lanthanum chloride can inhibit growth of human cervical cancer HeLa cells and induce cell apoptosis via Caspase pathway.
