HPLC-MS in simultaneous determination of 4 compounds in Fuzhengpingxiao capsule: Gentiopicroside, liquiritin, rosmarinic acid and harpagoside
10.3724/SP.J.1008.2011.00524
- Author:
Wen-Jun TIAN
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Fuzhengpingxiao capsule;
Gentiopicroside;
Harpagoside;
HPLC-MS;
Liquiritin rosmarinic acid
- From:
Academic Journal of Second Military Medical University
2011;32(5):524-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a HPLC-MS method to simultaneously determine the contents of gentiopicroside, liquiritin, rosmarinic acid and harpagoside in Fuzhengpingxiao capsule. Methods The chromatographic separation was performed on a Agilent Eclipse plus C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile (A)-0.1% methanoic acid (B) (0-30 min, 15% A-40% A), eluted at a flow rate of 1.0 ml/min and with a split ratio of 3: 1. Negative ionization and selected ion monitoring (SIM) mode on Mass were selected. Results The limit of quantitation (LOQ) values of gentiopicroside, liquiritin, rosmarinic acid and harpagoside were 5.73, 6.37, 6.50, and 6.46 ng/ml, and the limit of detection (LOD) values were 0.46, 0.32, 0. 33 and 0.32 ng/ml, respectively. Good linearity (r > 0.999 0) was obtained over the investigated concentration ranges. Reproducibility was evaluated by intra- and inter-day assays, and RSD values were below 2%. The average recoveries of the 4 compounds were within the range of 98%-102%. Conclusion We have established a rapid and efficient HPLC-MS method for determination of 4 constituents in Fuzheng pingxiao capsule: gentiopicroside, liquiritin, rosmarinic acid and harpagoside.
