Polymers poly (β-amino esters) nanoparticles for gene delivery
10. 3724/SP. J. 1008. 2011. 00473
- Author:
Jiang-Feng WANG
1
Author Information
1. Department of Pharmaceutics
- Publication Type:Journal Article
- Keywords:
Gene vectors;
Nanoparticles;
Polymers poly-amino ester;
Transfection efficiency
- From:
Academic Journal of Second Military Medical University
2011;32(5):473-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To synthesize a new type of cationic polymers (β-ammo esters) (PBAE) and to study its role as a gene vector. Methods: PBAE was synthesized by the Michael addition reaction. PBAE/pDNA complex nanoparticles were prepared by self-assembly, and the gene tranfection efficiency mediated by the nanoparticles into HEK293 cells in vitro was observed. Results: When weight ratio of PBAE/pDNA was 50: 1, pDNA was completely wrapped. And the transfection efficiency of PBAE/pDNA nanoparticles (PBAE/pDNA weight ratio being 200: 1) was significantly higher than PEI/pDNA nanoparticles (43.3% ± 3.7% vs 30.3% ± 2.1%, P < 00.05). Conclution: Cationic poly(β-amino esters) can electrostatically bind to and condense anionic DNA into nanometersized parties. PBAE/pDNA nanopartides has a high transfection efficiency in in vitro transfection experiments.
