Effect of ligustrazine hydrochloride injection on TGF-β1-induced proliferation and type IV collagen secretion in human mesangial cells
10.3724/SP.J.1008.2011.00633
- Author:
Xiao-Chun XUE
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Collagen type IV;
Ligustrazine hydrochloride;
Mesangial cells;
Transforming growth factor- β 1
- From:
Academic Journal of Second Military Medical University
2011;32(6):633-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ligustrazine hydrochioridc(LHC) injection on TGF-β1 -induced proliferation and type IV collagen secretion in the human mesangial cells(HMCs). Methods The interstitial fibrosis in kidney disease was mimicked by inducing proliferation and type IV collagen secretion in HMCs with TGF- β1. The experiment was divided into 5 groups: blank, control, low, medium, and high(10, 30, and 100 μg/ml)ligustrazinc hydrochloride groups. MTT method was adopted to examine the proliferation and inhibition rate of HMCs. Enzyme-linked immunosorbent assay was used to determine the production of type IV collagen in cultured HMCs. Results LHC at high concentration (100 μg/ml) significantly inhibited the proliferation of HMCs (P<0. 01). LHC also inhibited the production of type IV collagen, with the significant inhibition found when at the concentration of 100 μg/ml (P
