Changes of miRna-200c expression in ovarian cancer and its clinical significance
10.3724/SP.J.1008.2011.00612
- Author:
Lin-Xia LI
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- Keywords:
Micrornas;
Ovasian neoplasms;
Prognosis
- From:
Academic Journal of Second Military Medical University
2011;32(6):612-616
- CountryChina
- Language:Chinese
-
Abstract:
Objeciive To observe the expression of microRNA-200c (miR-200c) n ova]iian cancer cell ine and tissues, and to assess its possible ctinical Value. Methods Real-time PCR was Used to analyze the expression of miR-200c and miR-141 in HO-8910 (hijman oVarian cancer cell inn-e), HO-8910PM (highly metastatic ovaiiian cancer cell ine), and HO-8910 cluster cells. We also determined the expression of miR-200c and miR-141 in 83 orarian cancer tissues, 13 borderitine ovaiiian tijmors, and 8 normal ovary tissties; the expression was compared between tijmors of different pathological character Resuits Expression of miR-200c Was down-regulated in HO-8910 cluster cells compared with those n HO-8910 and HO-8910PM cells (P<0 05). Expressions of miR-141 and miR-200c were gradually Up-regulated from the normal ovary tissUe to borderiine ovasiari tirmors, then to ovarian cancer tissties (P
