Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP
10.3724/SP.J.1008.2011.00607
- Author:
Zhen DENG
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Neoplasm invasiveness;
Prostaiic neoplasms;
miR-29c;
cell proliferasion
- From:
Academic Journal of Second Military Medical University
2011;32(6):607-611
- CountryChina
- Language:Chinese
-
Abstract:
Objedive To observe the effect of microRNA-29c cm the proliferalion and invasion abililies of hirman prostate carreer cell litre LNCaP, and to discijss its potetrlial applicasion. Methods The difference in miR-29c expression between ADPC (androgen-dependent prostate cancer) and AIPC (androgen-independent prostate cancer)cell lines was observed by real time RT-PCR.MiR-29c-inhibitor (anti-miR-29c) was used to decrease miR-29 cexpression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c.Results The result of real-timeRT- PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells (P<0.05).Down- expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells (P<0.05). Conclusion Normal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells, in dicating it might be a new target for biotherapy of prostate cancer.
