Tanshinone II A induces apoptosis in human nasopharyngeal carcinoma CNE1 cells
10.3724/SP.J.1008.2011.00879
- Author:
Jian ZHAO
1
Author Information
1. Department of Otorhinolaryngology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Bcl-2;
Cell cycle;
Cyclooxygenase 2;
Nasopharyngeal neoplasms;
Tanshinone
- From:
Academic Journal of Second Military Medical University
2011;32(8):879-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of anshinone II A (Tan II A) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and one xpression of Bcl-2 gene and COX-2 promoter. Methods CNE1 cells were Cultured in vitro and treated with various concentrations of Tan II A. The cytotoxicity effect of Tan II A against CNE1 cells was evaluated by MTT assay. The cell cycle distribution, apoptotic rate and Bcl-2 expression were investigated by flow cytometry. COX-2 promoter activity was detected by the dual luciferase assay. Results Tan II A treatment inhibited CNE1 cell proliferation In a concentration- And time-dependent manner (P<0.01). Compared with the control group, Tan II A groups had significantly higher apoptosis rates (P<0.01), and the apoptosis rates increased with the increase of Tan II A concentration; the cells were blocked in the G2/M phase. Tan II A inhibited Bcl-2 protein expression in a concentration- dependent manner with in 6 h after Treatment (P<0.05). Bcl-2 expression was initially increased and then decreased with the increase of T an II A concentrations (P<0.05) 24 h after treatment. Activity of COX -2 promoter decreased with the increase of Tan II A concentration (P<0.01). Conclusion Tan II A can inhibit the growth and promote the apoptosis of CNE1 cells; inhibition of Bcl-2 expression and COX-2 activity might be one of the mechanisms for the Tan II A-induced CNE1 cell apoptosis.
