Impact of ulinastatin on survival and lymphocyte apoptosis in septic mice
- Author:
Na HUANG
1
Author Information
1. Department of Anesthesiology
- Publication Type:Journal Article
- Keywords:
Sepsis;
ulinastatin;
cecal ligation and puncture;
lymphocyte count;
apoptosis
- From:
Academic Journal of Second Military Medical University
2011;32(8):869-873
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of ulinastatin on the survival and lymphocyte apoptosis in mice with cecal ligation and puncture(CLP) induced sepsis, so as to investigate the possible mechanism of ulinastatin action in sepsis. MethodsForty 8-10 weeks old Methods Forty8?10weeksoldmaleC57BL/6mice(22?30gbodyweight)wererandomlyassignedtofourgroups,i.e.,Sham group,CLP group,CLP plus UTI ip group (100000U/kg by intraperitoneal injection at half an hour after operation),and CLP plus UTI iv group(100000U/kg by intravenous injection at half an hour after operation).After success ful establishment of CLP model,the7-daysurvivalofthe10animalsineachgroupwasobservedandthesurvivalcurvewasalsoplotted.Basedon the 7-day survival of animals,another 40 mice were divided into the above 4 groups and specimens were collected 24h after CLP to examine the lymphocyte count in the peripheral blood, thymus and spleen and theapoptosis of lymphocytes in the thymus and spleen.The statistical analyses were done with Prism 5.01(Graph Pad Software, USA).Results The 7-day survival ratesin the two UTI groups were significantly higher than that in the CLP group(P<0.05).The lymphocyte count sin the two UTI groups were significantly higher than that in the CLP group (P<0.05),and the lymphocyte apoptosis rates in the two UTI groups were significantly lower than that in the CLP group(P<0.05).The above parameters were not significantly different between the two UTI groups.Conclusion Both intraperitoneal and intravenous treatment with UTI can greatly improve the survival of septic mice,which might be related to the roles of UTI inincreasing the lymphocytes count in the peripheral blood, thymus and spleen of septic mice and in alleviating lymphocyte apoptosis in the thymus and spleen.
