Identification and bioactivities analysis of Halomonas sp. from seawater
10.3724/SP.J.1008.2011.00813
- Author:
Gui-Cheng ZHAO
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- Keywords:
Antioxidants;
Cytotoxins;
Halomonadaceae;
Identification;
Leukocyte elastase
- From:
Academic Journal of Second Military Medical University
2011;32(8):813-817
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the 16S rDNA sequences of 36 strains of Halomonas sp. Isolated from the seawater of the East China Sea and to study their bioactivities. Methods: The 16S rDNA sequences of all isolated strains were amplified by PCR, and the obtained sequences were subjected to phylogenetic analyses using MEGA4.1 and multiple sequence alignment with Clustal X1. 8 software. MTT assay was applied to assess the cytotoxic activities of their fermentation broth; ABTS and DPPH antioxidant models were applied to test the antioxidation activities of their fermentation broth. The HLE inhibition activities of the fermentation broth were tested by a stable HLE in hibitor screening model. Results: Analysis of 16S rDNA sequence showed that the 36 strains had a high affinity (96%-99%) to the Halomonas sp. Fermentation broth of the 36 strains showed different degrees of cytotoxic activities, antioxidation activities, and HLE inhibition activities. Eleven strains inhibited the growth of HepG2 cells, with the inhibition rate ranging (4.40±1.2)%to(24.90±3.5) %; 20 strains inhibited the growth of HL-60 cells, with the inhibition rate ranging (1.70±1.1)% to (50.90±4.2) %; 7 strains showed ABTS radical scavenging capacity, with the scavenging rate ranging (4.49±2.1) % to (58.43±4.4) %; 3 strains showed DPPH radical scavenging capacity, with the scavenging rate ranging (5.68±3.7) % to (59.06±3.2) %, and 3 strains showed HLE inhibition activities, with the inhibition rate ranging (12.71±1.81)% to (71.19±5.62) %. Conclusion: All the 36 Halomonassp. Strains show different degrees of cytotoxic effects, antioxidation activities, and HLE inhibition capacities. Several strains with high activities possess the potential for medicinal application.
