Loss of heterozygosity at chromosome 3p microsatellite loci and angiogenesis in breast cancer
10.3724/SP.J.1008.2011.00979
- Author:
Feng QIAO
1
Author Information
1. Department of Thyroid and Breast Surgery
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
Breast neoplasms;
Loss of heterozygosity;
Vascular endothelial growth factor
- From:
Academic Journal of Second Military Medical University
2011;32(9):979-984
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association of loss of heterozygosity (LOH) at chromosome 3p and vascular endothelial growth factor (VEGF) expression with clinicopathological features of breast cancer. Methods A total of 40 specimens of breast invasive ductal carcinoma were analyzed in the present study. The ductal carcinoma cells and normal breast cells were microdissected from the paraffin sections. Capillary electrophoresis method was used to detect LOH of four microsatellite loci located in the 3p site. Immunohistochemical staining was used to investigate the expression of CD34, VEGF and Her2 proteins in the 40 specimens. The association of LOH with the clinicopathological characteristics of breast carcinoma was evaluated. Results We found that in the 40 specimens LOH of D3S1038 was 20%(8/40), LOH of D3S1295 was 37.5% (15/40), LOH of D3S1581 was 17.5% (7/40), and LOH of D3S3118 was 5% (2/40). LOH was positively correlated with expression of VEGF (r=0.658 0, P<0.01) and MVD (r=0.804 9, P<0. 01). We also found that LOH was significantly related to Her2 (r=0.539 4), differentiation (r=0.497 2), lymph node metastasis(r = 0. 506 4), and TNM stage(r = 0.596 0, all P<0.01). Conclusion LOH at chromosome 3p microsatellite loci is correlated with angiogenesis. Inactivation of tumor suppressor may influence angiogenesis and subsequently promote tumor growth and metastasis. Detection of LOH at chromosome 3p microsatellite loci together with angiogenesis can help to fully understand the biological characteristics of breast cancer.
