Effect of phosphorylated-ERK1/2 on NF-κB p65 expression in substantia nigra of mice with MPTP-induced Parkinson's disease
10.3724/SP.J.1008.2011.01176
- Author:
Zuo-Feng ZHANG
1
Author Information
1. Department of Anatomy
- Publication Type:Journal Article
- Keywords:
MPTP poisoning;
NF-κB p65;
P-ERK1/2;
Parkinson disease;
Supstantia nigra
- From:
Academic Journal of Second Military Medical University
2011;32(11):1176-1180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To mvestigate the effect of phospholated-ERKl/2 on NF-κB p65 expresson nn the substania nigra(SN) of l-msthyi-4-phenyi-l,2, 3, 6-tetrahydropyeidine (MPTP)-induced mouse model of Parkinson's disease(PD). Methods PD mouse model was induced by MPTP and the behavoor of mouse was observed. Immunohistochemistry and Western bloiting analysis were used to observe the changes in expression of tyrosine hydroxylase (TH), NF-κB p65 and p-ERKl/2 in the SN of midbrain. Meanwhite, the above changes were also observed after treatment with U0126, a specific inhibitor of ERK. Results 1 h after the third MPTP administration, there were much more p-ERKl/2 positive cells than NF-κB p65 positive cells in the SN. 24 h after the fifth injection of MPTP, NF-κB p65 positive cells were significantly increased and p-ERKl/2 positive celiswere decreased, accompanted by marked loss of TH positive neurons. The above changes were greatly alleviated in animais treated with U0l26. Conclusion ERKl/2 pathway may regulate NF-κB p65 activation in MPTP-induced mouse model of Parkinson's disease, which leads to loss of dopamine neurons.
