Protective effect of atorvastatin against myocardial ischemia-reperfusion injury in rats
10.3724/SP.J.1008.2012.01070
- Author:
Hui YU
1
Author Information
1. Department of the Cardiovasology, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Atorvastatin;
Nitric oxide synthase;
Reperfusion injury;
Tumor necrosis factor alpha
- From:
Academic Journal of Second Military Medical University
2012;33(10):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of atorvastatin against myocardial ischemia-reperfusion injury in rats and to discuss the possible mechanism. Methods Thirty-six healthy male Sprague-Dawley rats were evenly randomized into three groups: sham-operated (S) group, ischemia-reperfusion (I/R) group, and atorvastatin pretreatment (AT+I/R) group. The myocardial ischemia-reperfusion model was established by ligating the left anterior descending of coronary artery. The infarcted area was evaluated by Evans blue and triphenyltetrazolium chloride (TTC) staining. The contents of tumor necrosis factor alpha (TNF-«), malondialdehyde (MDA), and myeloperoxidase (MPO) and total superoxide dismutase(TSOD) activity in the non-infarcted myocardial tissues were measured by radioimmunoassay; the levels of nitric oxide (NO) and the activities of nitric oxide synthase (NOS) and inducible NOS(iNOS) were detected by colorimetric method. Results The ratio of the infarcted area to the ischemia area (ischemia but non-infarcted area+infarcted area) and the ratio of the infarcted area to the left ventricular area in group AT+I/R were both significantly lower than those in group I/R ([29. 4±8. 4) % vs [57. 7±6. 5]%, P<0. 001; [15. 9±5. 6]% vs [29. 0±8. 9]%, P<0. 05). The levels ofTNF-«, MDA, MPOand NO, the activities ofNOS and iNOS were significantly higher and the TSOD activity was significantly lower in the non-infarcted myocardial tissues of group AT+I/R and group I/R compared with those in group S (P<0. 05). The levels of TNF-«, MDA, MPO and NO, and the activities of NOS and iNOS were significantly decreased (P<0. 05) and the TSOD activity was significantly increased (P< 0. 05) in group AT+I/R as compared to those in group I/R. Conclusion Atorvastatin can protect myocardium from ischemiareperfusion injury, which might be related to suppression of the inflammatory reaction, activation of the anti-oxidiant reaction and improvement of oxygen free radical scavenging; the role of iNOS deserves special attention.
