Effects of angiotensin II inhibitor on HIF-1α expression in the renal tissue of 5/6th kidney ablation/infarction rats
10.3724/SP.J.1008.2012.00965
- Author:
Jun-yan QIN
1
Author Information
1. Department of Nephrology, Shuguang Hospital, Shanghai University of TCM
- Publication Type:Journal Article
- Keywords:
Alpha subunit;
Angiotensin II;
Anoxia;
Hypoxia-inducible factor 1;
Kidney failure;
Renal fibrosis
- From:
Academic Journal of Second Military Medical University
2012;33(9):965-969
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of Angiotensin II inhibitor on renal expression of HIF-1α mRNA and protein in the chronic renal failure (CRF) rats (induced by 5/6th kidney ablation/infarction \[5/6A/I\]) and the related mechanism. Methods SD rats were made into CRF model by means of 5/6th kidney ablation/infarction. The study was divided into sham operation group (Group A), model group (Group B) and Angiotensin II inhibitor(Cozaar-with-Monopril) group (Group C), each group containing 15 rats. The biochemical parameters of the rats were examined 60 days later in the 3 groups. The expression of HIF-1α mRNA and protein in the remnant renal cortex and medulla were assessed by Real-time PCR and Western blotting analysis. Results Compared with group A, group B had significantly increased SCr and BUN levels and significantly decreased CCr level(P<0.01). Compared with group B, group C had significantly decreased SCr and BUN levels and significantly increased CCr level(P<0.05). The expression of HIF-1α mRNA and protein in the renal cortex and medulla in group B was significantly higher than that in group A(P<0.05), and that in group C was significantly lower than that in group B (P<0.05). Conclusion Angiotensin II inhibitor can improve the renal function in CRF rats induced by 5/6(A/I), which might be associated with HIF-1α signaling pathway.
