Establishment of a fluorescence-labeled human ovarian cancer cell line
10.3724/SP.J.1008.2012.00950
- Author:
Xiao-mei ZHANG
1
Author Information
1. Department of Biomedical Engineering, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Green fluorescent proteins;
HO8910PM cell line;
Ovarian neoplasms;
Transfection
- From:
Academic Journal of Second Military Medical University
2012;33(9):950-954
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a human ovarian cancer cell line stably expressing enhanced green fluorescent protein(EGFP), so as to carry out visualized research on whole ovarian cancer. Methods We transfected human ovarian cancer cell line HO8910PM by gene transfection, and obtained cells stably expressing EGFP by sub-cloning amplification and selection with G418-resistance.The expression rate of EGFP was analyzed by flow cytometry (FC).The growth curve, adhesion, migration and invasion experiments were employed to study the biological behaviors of the cells transfected with EGFP. Results Flow cytometry results showed that EGFP positive rate of screened EGFP-HO8910PM cells was higher than 99%. The cell growth, adhesion, invasion and migration abilities of cells were not significantly changed after transfection. Conclusion We have successfully established a cell line EGFP-HO8910PM stably expressing EGFP and at mean time maintaining the characteristics of the parent cell line, which lays a foundation for whole-body visualization research of human ovarian cancer in vivo.
