Effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma cell line SGC-7901
10.3724/SP.J.1008.2012.00851
- Author:
Jie ZHAO
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
Cell Migration;
Cell proliferation;
S100A7;
Small interfering RNA;
Stomach neoplasms
- From:
Academic Journal of Second Military Medical University
2012;33(8):851-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma SGC-7901 cells, and to explore the possible molecular mechanisms. Methods The experiment was divided into three groups, including untransfected group, control siRNA group and S100A7 siRNA group. S100A7 siRNA and control siRNA were transfected into gastric carcinoma SGC-7901 cells in S100A7 siRNA group and control siRNA group, respectively. Expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells were examined by Real-time PCR and Western blotting analysis, respectively. CCK-8 kit and Boyden chamber were used to observe the proliferation and migration of SGC-7901 cells in the three groups. The expressions of cyclin Dl, Cdk2 and MMP-2 proteins were also examined by Western blotting analysis in the three groups. Results S100A7 siRNA effectively down-regulated the expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells. Down-regulated S100A7 expression greatly inhibited the proliferation and migration of gastric carcinoma SGC-7901 cells, and also down-regulated expression of cyclin Dl, Cdk2 and MMP-2 proteins. Conclusion Down-regulated S100A7 expression-mediated inhibition of gastric carcinoma cell proliferation and migration may be closely associated with the decreased expressions of cyclin Dl, Cdk2 and MMP-2 proteins.
