Expression of ZEB-1 in hepatocellular carcinoma tissues and its significance
10.3724/SP.J.1008.2012.00842
- Author:
Xiao-yu YANG
1
Author Information
1. Department of Special Treatment II, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Immunohistochemistry;
Liver neoplasms;
Paracancerous tissue;
ZEB-1
- From:
Academic Journal of Second Military Medical University
2012;33(8):842-845
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate ZEB-l protein expression in hepatocellular carcinoma (HCC) tissues and the relevant significance. Methods Immunohistochemical method and Western blotting analysis were used to detect the ZEB-l protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues. The relationship between ZEB-l protein expression and clinicopathological features of HCC was evaluated. Results Immunohistochemical staining showed that the positive rates of ZEB-l protein expression in HCC tissues and paracancerous cirrhotic tissues were 93. 5% (58/ 62) and 83.3 (40/48), respectively. Western blotting analysis showed that ZEB-l protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues (P<0. 05). We also found that ZEB-l expression was significantly correlated with the clinical stage, tumor differentiation degree, metastasis, portal vein tumor thrombus and postoperative recurrence (P<0. 05), and was not correlated with the tumor diameter, number of tumors, or serum AFP levels (P>0. 05). Conclusion High expression of ZEB-l is correlated with the development and progression of HCC. ZEB-l might also participate in the epithelial-mesenchymal transition of HCC cells, subsequently contributing to the invasion and metastasis.
