Thyroid hormone preconditioning alleviates reperfusion-induced renal inflammation in mice
10.3724/SP.J.1008.2012.00364
- Author:
Pan-Liang WANG
1
Author Information
1. Department of Liver Surgery, Renji Hospital, Shanghai Jiaotong University, School of Medicine
- Publication Type:Journal Article
- Keywords:
Interleukin-1 receptor antagonist;
Interleukin-10;
Kidney;
Reperfusion injury;
Triiodothyronine
- From:
Academic Journal of Second Military Medical University
2012;33(4):364-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of thyroid hormone T3 preconditioning on interleukin-10 (IL-10) and interleukin-1 receptor antagonist (IL-IRa) modulation and neutrophil infiltration after renal ishchemia/reperfusion (IR) in mice, so as to study the protective effect of T3 on IR kidney. Methods Totally 120 male C57BL/6 mice were randomly divided into four groups (n = 30), namely, control group (sham operation), IR group (only received renal IR), T3 + IR group (T3 preconditioning for 48 h before renal IR), and NaOH + IR group (received equivalent 0. 1 mol/L NaOH soltuion 48 h before renal IR). The serum creatinine and blood urea nitrogen (BUN) were determined 24 h after reperfusion in each group; renal histological damages were scored using PAS staining; the levels of neutrophil infiltration were evaluated by MPO staining, and IL-10, IL-IRa mRNA expression was examined by real-time PCR at 1, 3, 6, 12, 24, and 48 h after reperfusion. Results The serum creatinine and BUN levels of T3+IR group were significantly lower than those of IR group 24 h after reperfusion (P< 0. 05), which was accompanied by lower histological score and significantly less neutrophil infiltration (P<0. 05). Real-time PCR results showed that IL-10 and IL-IRa mRNA expression in T3 +IR group was significantly higher than that in the IR group (P<0. 05) 12 h after reperfusion, which lasted for 48 h aft er reperfusion. The above parameters were similar between IR group and NaOH+IR group. Conclusion Thyroid hormone T3 preconditioning can alleviate renal IR injury, partly by increasing expression of IL-10 and IL-IRa and subsequently reducing neutron phil infiltration at the late phase of renal IR.
