Artesunate induced G2/M phase cell cycle arrest in multiple myeloma RPMI8226 cells
10.3724/SP.J.1008.2013.00794
- Author:
Su-Yun WANG
1
Author Information
1. Department of Hematology, Hebei General Hospital
- Publication Type:Journal Article
- Keywords:
Artesunate;
CDC2 protein kinase;
Cell cycle;
Cyclin B1;
Multiple myeloma
- From:
Academic Journal of Second Military Medical University
2013;34(7):794-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of artesunate on the cell cycle of human multiple myeloma RPMI8226 cells and explore the related molecularmechanisms. Methods RPMI8226 cellswere treated with 0, 25, and 50 pg/mL artesunate. The morphology change of cells was observed under transmission electron microscope, the cell cycle distribution was detected by flow cytometry, and the expression of cyclin B1 and p34cdc2 protein was examined by Western blotting analysis. Results After treated with 50 pg/mL artesunate for 48 h, most RPMI8226 cells showed characteristic morphology of apoptosis. With the increase of artesuate concentration, the proportion of RPMI8226 cells in G0/G1 phase was significantly decreased (P<0. 05) and that of cells in G2/M phase was significantly increased (P<0.05), suggesting that artesunate induced noticeable G2/M arrest. Cyclin B1 level was increased and the p34cdc2 level was decreased with the increase of artesuate concentration. Conclusion Artesunate can induce G2/M arrests in RPMI8226 cells, which may be related to the increasedcyclin B1 expression and decreased p34cdc2 expression.
