Effect of melatonin receptor agonist Neu-Pll on expression of IRS-1 and GLUT-4 in insulin-resistant adipocytes
10.3724/SP.J.1008.2013.00561
- Author:
Xiu-Ping LI
1
Author Information
1. Department of Laboratory Medicine, Huaihua Medical College
- Publication Type:Journal Article
- Keywords:
Adipocytes;
High glucose/high insulin;
Insulin resistance;
Melatonin;
Neu-P11
- From:
Academic Journal of Second Military Medical University
2013;34(5):561-564
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of melatonin receptor agonist Neu-P11 on the expression of IRS-1 and GLUT-4 in insulin-resistant 3T3-L1 adipocytes. Methods Insulin resistant 3T3-L1 adipocytes were induced with high glucose/ high insulin for 24 hours, and then they were divided into 4 treatment groups; melatonin, Neu-P11, melatonin+luzindole and Neu-P11 + luzindole. And non-treated insulin-resistant 3T3-L1 adipocytes were taken as control. Glucose consumption was detected by enzymatic method. IRS-1 and GLUT-4 protein expressions were detected by Western blotting analysis. Results After the insulin-resistant adipocytes were treated with melatonin and Neu-P11, the glucose consumption andthe expressions of IRS-1, GLUT-4 proteinswere significantly increased compared with the non-treated control group (P<0. 05). The expressions of IRS-1, GLUT-4 proteins in melatonin + luzindole (melatonin receptor antagonist) and Neu-P11 + luzindole groups were significantly decreased compared with melatonin alone or Neu-P11 alone groups (P<0. 05), and were similar to those in thenon-treated control group. Conclusion Melatonin receptor agonist Neu-P11 can increase glucose consumption, insulin sensitivity in insulin resistant-adipocytes, which might be associated with the up-regulation of IRS-1 and GLUT-4 protein expression.
