Expression of UBE1 and Bcl-2 in diffuse large B cell lymphoma and its clinical significance
10.3724/SP.J.1008.2013.00295
- Author:
Bing CHEN
1
Author Information
1. Department of Pathology, Wannan Medical College
- Publication Type:Journal Article
- Keywords:
Bcl-2 protein;
Diffusse large B-cell lymphoma;
Ubiquitin-activating enzyme E1
- From:
Academic Journal of Second Military Medical University
2013;34(3):295-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of ubiquitin-activating enzyme E1 (UBE1) and Bct-2 in diffuse large B cell lymphoma (DLBCL) and its clinicopathological relevance. Methods Tumor tissues were collected from 80 DLBCL patients, and tissues from 30 patientswith reactive lymphoid hyperplasia (RH) were taken as controls. Immunohistochemistry detection (EnVision method) was used to detect the expressions of CD10, Bct-6 and MUM1 inDLBCL tissues for immunology typing of GCB or ABC. The expression of UBE1 and Bct-2 proteins in DLBCL tissues and RH tissues was assayed by immunohistochemical staining, and its correlation with patients' clinical pathological indices were analyzed. We also analyzed the correlation of UBE1 expression with Bct-2 expression in DLBCL tissues. Results The positive rates of UBE1 were 65. 0% (52/80) in DLBCL tissues and 13. 3%(4/30) in RH tissues (P<0. 05). The positive rates of UBE1 were 53. 3% (16/30) for GCB type DLBCL and 72. 0%(36/50) for ABC type (P<0. 05). The positive rates of Bct-2 were 51. 3% (41/80) in DLBCL tissues and 10. 0% (3/30) in RH tissues (P<0. 05). The positive rates of Bcl-2 were 36. 7%(11/30) for GCB type DLBCL and 60. 0% (30/50) for ABC type (P<0. 05). The positive rate of UBE1 was correlated with age and the extranodal involvement site (P<0. 05). The positiverate of Bcl-2 was correlated with age, Ann Arborstage, extranodal involvement site and international prognostic index (P<0. 05). UBE1expression was positively correlated with Bcl-2 in ABC type DLBCL (r =0.582, P = 0. 001). Conclusion UBE1 and Bcl-2 proteins are highly expressed in ABC type DLBCL tissues, which probably plays a role in the development and progression of DLBCL. High expression of UBE1 and Bcl-2 may indicate poor prognosis of DLBCL patients.
