Resveratrol protects against bleomycin-induced lung injury in mice
10.3724/SP.J.1008.2013.01287
- Author:
Hong-Xia ZHANG
1
Author Information
1. Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongli University School of Medicine
- Publication Type:Journal Article
- Keywords:
Bleomycin;
Inflammation;
Lung injuries;
Oxidative stress;
Resveratrol
- From:
Academic Journal of Second Military Medical University
2013;34(12):1287-1290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effects of the resveratrol on bleomycin-induced lung injury in mice and its underlying mechanism. Methods Male ICR mice were randomly divided into four groups (n = 10): control group, bleomycin group, resveratrol group, and bleomycin+resveratrol group. Micewere anesthetized and infused intratracheally with 3 mg/kg bleomycin to create lung injury model. Resveratrol (0. 3 mg/kg) was administered intraperitoneally before bleomycin infusion. The severity of pulmonary injuries was evaluated 24 h after inducing lung injury. H-E staining was used to assess the lung pathology. The levels of lipid peroxidation marker malondialdehyde (MDA), neutrophil infiltration marker myeloperoxidase (MPO) activity, and cytokines IL-6, IL-8, and IL-10 were determined by commercial kits. Results Resveratrol treatment improved the bleomycin-induced lung injury in mice by significantly inhibiting the oxidative stress and decreasing lipid peroxidation marker MDA level (P<0. 01). Resveratrol treatment also attenuated the bleomycin-induced pulmonary infection by significantly decreasing MPO activity (P<0. 01) and the production of pro-inflammatory cytokines IL-6 (P<0. 01) and IL-8 (P< 0. 05) and increasing anti-inflammatory cytokine IL-10 production (P<0. 01). Conclusion Resveratrol can protect against bleomycin-induced lung injury in mice, probably through its antioxidant and anti-inflammatory effects.
