Peri-Operative bile metabonomics changes of extrahepatic cholangiocarcinoma and prognosis analysis
10.3724/SP.J.1008.2015.00383
- Author:
Long-Jiu CUI
1
Author Information
1. Department of Special Treatment II, Eastern Hepatobliary Surgery Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Extrahepatic cholangiocarcinoma;
Gas chromatography-mass spectrometry;
Metabolomics;
Principal component analysis
- From:
Academic Journal of Second Military Medical University
2014;36(4):383-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for molecular markers associated with the prognosis of extrahepatic cholangiocarcinoma (EHCC) after radical excision by observing the difference of bile metabolomics during perioperative period. Methods Twenty-seven patients with EHCC, including 19 with hilar cholangiocarcinoma and 8 with distal cholangiocarcinoma, were included in this study. Their bile samples were collected before and 1, 7 days after operation. Metabonomics method using gas chromatograph^mass spectrometry (GC-MS) was employed to analyze the different metabolites at different time points. Correlation analysis was performed between the common different metabolites and the patient prognosis. Results Five kinds of metabolites in the bile were notably increased 1 day after operation compared with that before operation, no metabolites were decreased. Three kinds of metabolites were notably increased and another 3 were notably decreased 7 days after operation compared with before operation. Among the above two groups of different metabolites, there were 2 common metabolites: benzoic acid and methylmalonic acid. The one year recurrence rate of patients with benzoic acid increased ≥2 times at 7 days after operation was significantly lower than that increased <2 times (P= 0. 04). No significant correlation was found between the change of methylmalonic acid and the early relapse in 1 year after operation. Moreover, bivariate correlation showed that the changes of benzoic acid metaboilic level in bile were not significantly correlated with the serum CA19-9, age of patients, or TNM stage (P>0. 05). Conclusion The change of benzoic acid metaboilic level in bile during perioperative period can help to predict the early relapse in extrahepatic cholangiocarcinoma patients after curative resection.
