Epithelial mesenchymal transition during the development of ductal plate in human liver and its significance
10.3724/SP.J.1008.2014.01378
- Author:
Jun XU
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Developing human liver;
Ductal plate;
Epithelial mesenchymal transition;
Hepatic stem cells;
Immunohistochemistry
- From:
Academic Journal of Second Military Medical University
2014;35(12):1378-1381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of markers for epithelial-mesenchymal transition (EMT) during the development of ductal plate in human liver, so as to discuss the role of EMT during ductal plate development in the liver. Methods Immunohistochemical method was used to examine the expression of EMT markers (CK19, vimentin, and ccSMA) in the liver tissues of 31 fetuses of 8-40 weeks old. Results From the 8th gestation week onwards (ductal plate phase, remodeling phase of ductal plate, and formation phase of bile ducts), the CK19-positive cells in the portal tract were gradually increased, vimentin/ ccSMA-positive portal mesenchymal cells were gradually decreased, and CK19-positive cells were negatively correlated with vimentin/ ccSMA expression ones (vimentin: r=- 0. 820, P
