Transplantation of autologous angiogenin-overexpressing marrow stromal cells in treatment of a porcine chronic ischemia model
10.3724/SP.J.1008.2014.00586
- Author:
Xun-Yu XU
1
Author Information
1. Department of Thoracic Surgery, Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Angiogenin;
Cell transplantation;
Ischemic heart disease;
Marrow stromal cells
- From:
Academic Journal of Second Military Medical University
2014;35(6):586-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of autologous angiogenin(ANG)-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia. Methods: Porcine models of chronic ischemia were established and were randomized into the following 3 groups: autologous marrow stromal cells transfected with Ad-ANG (Group I, n=11), autologous marrow stromal cells without transfection (Group II, n=10), and controls injected with serum-free DMEM medium (Group III, n=10). The autologous marrow stromal cells were cultured and were transfected with Ad-ANG, and the cells were labeled by CM-Dil. The cels were transplanted to the ischemia site of porcine heart. The parameters we observed included Rentrop score, ejection fraction, percentage of infracted area, CM-Dil labeled cells under fluorescence microscope, number of blood vessels and expression of ANG protein. Results: Compared with group H and IE, Group I had significantly increased Rentrop score, ejection fraction, numbers of blood vessels in the infarcted areas, CM-DiI labeled cells, and ANG protein expression, but significantly decreased infarcted area (P < 0. 05 or P < 0. 01). Conclusion: Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells, greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.
