Single-pass intestinal perfusion for studying intestinal absorption of azithromycin niosomes in rats
10.3724/SP.J.1008.2014.0419
- Author:
Meng ZHONG
1
Author Information
1. Medicine Engineering Research Center, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Azithromycin;
Intestinal absorption;
Niosomes;
Single-pass intestinal perfusion
- From:
Academic Journal of Second Military Medical University
2014;35(4):419-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of azithromycin niosomes (AM-NS) in promoting the intestinal absorption of AM in rats. Methods Six healthy male rates were randomly divided into two groups. Single-pass intestinal perfusion was employed to investigate the intestinal absorption of the duodenum, jejunum, ileum and colon when the perfusion rate was 0. 2 mL/min and period was 1 h. The concentrations of the drugs were determined by HPLC; the drug absorption rate constant (Ka) and the intestinal apparent permeation coefficients (Peff) were calculated. Results The absorption Ka of AM-NS in the duodenum, jejunum, ileum and colon were 2. 41, 2. 35, 2. 54 and 2. 95 folds that of the free AM, and the Peff were 2. 34, 1. 47, 2. 20, and 1. 40 folds that of the free AM, respectively, with significant differences found between the two groups for both Ka and Peff (P<0. 05). Conclusion AM-NS can significantly improve the absorption of azithromycin in the intestine of rats.
