Luteolin 5-O-glucoside from Korean Milk Thistle, Cirsium maackii, Exhibits Anti-Inflammatory Activity via Activation of the Nrf2/HO-1 Pathway.
10.20307/nps.2017.23.3.183
- Author:
Hyun Ah JUNG
1
;
Anupom ROY
;
Qudeer Ahmed ABDUL
;
Hyeung Rak KIM
;
Hee Juhn PARK
;
Jae Sue CHOI
Author Information
1. Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 54896, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Luteolin 5-O-glucoside;
Cirsium maackii;
Anti-inflammation;
HO-1/Nrf-2;
NF-κB;
λ-carrageenan
- MeSH:
Animals;
Body Weight;
Cirsium*;
Edema;
In Vitro Techniques;
Luteolin*;
Mice;
Milk Thistle*;
Milk*;
Nitric Oxide;
RAW 264.7 Cells
- From:Natural Product Sciences
2017;23(3):183-191
- CountryRepublic of Korea
- Language:English
-
Abstract:
Luteolin 5-O-glucoside is the major flavonoid from Korean thistle, Cirsium maackii. We previously reported the anti-inflammatory activities of luteolin 5-O-glucoside in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In this study, we determined the anti-inflammatory mechanisms of luteolin 5-O-glucoside through the inhibition of nitric oxide (NO) production in vitro and in vivo. Results revealed that luteolin 5-O-glucoside dose-dependently inhibited NO production and expression of iNOS and COX-2 in LPS-induced RAW 264.7 cells. Luteolin 5-O-glucoside also significantly inhibited the translocation of NF-κB, the activation of MAPKs, and ROS generation in LPS-induced RAW 264.7 cells. In addition, protein expressions of Nrf-2 and HO-1 were also upregulated by luteolin 5-O-glucoside treatment. Moreover, luteolin 5-O-glucoside inhibited λ-carrageenan-induced mouse paw edema by 65.34% and 48.31% at doses of 50 and 100 mg/kg body weight, respectively. These findings indicate potential anti-inflammatory effect of luteolin 5-O-glucoside particularly by downregulating NF-κB and upregulating HO-1/Nrf-2 pathway.
