Effects of Nodakenin, Columbianadin, and Umbelliferone Isolated from the Roots of Angelica decursiva on the Gene Expression and Production of MUC5AC Mucin from Human Airway Epithelial NCI-H292 Cells.
10.20307/nps.2017.23.3.201
- Author:
Hyun Jae LEE
1
;
Choong Jae LEE
Author Information
1. Department of Health Management and Smith Liberal Arts College, Sahmyook University, Seoul 01795, Korea.
- Publication Type:Original Article
- Keywords:
Mucin;
Nodakenin;
Columbianadin;
Uumbelliferone
- MeSH:
Angelica*;
Enzyme-Linked Immunosorbent Assay;
Epidermal Growth Factor;
Epithelial Cells;
Gene Expression*;
Humans*;
Lung Diseases;
Medicine, Traditional;
Mucins*;
Necrosis;
Polymerase Chain Reaction;
Reverse Transcription
- From:Natural Product Sciences
2017;23(3):201-207
- CountryRepublic of Korea
- Language:English
-
Abstract:
Angelica decursiva has been utilised as remedy for controlling the airway inflammatory diseases in folk medicine. We investigated whether nodakenin, columbianadin, and umbelliferone isolated from the roots of Angelica decursiva inhibit the gene expression and production of MUC5AC mucin from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with nodakenin, columbianadin or umbelliferone for 30 min and then stimulated with epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-α (TNF-α) for 24 h. The MUC5AC mucin gene expression was measured by reverse transcription - polymerase chain reaction (RT-PCR). Production of MUC5AC mucin protein was measured by enzyme-linked immunosorbent assay (ELISA). The results were as follows: (1) Nodakenin did not affect the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α. Columbianadin inhibited the expression of MUC5AC mucin gene induced by EGF or PMA. However, umbelliferone inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α; (2) Nodakenin also did not affect the production of MUC5AC mucin protein induced by EGF, PMA or TNF-α. Columbianadin inhibited the production of MUC5AC mucin protein induced by PMA. However, umbelliferone inhibited the production of MUC5AC mucin protein induced by EGF, PMA or TNF-α. These results suggest that, among the three compounds investigated, umbelliferone only inhibits the gene expression and production of MUC5AC mucin stimulated by various inducers, by directly acting on airway epithelial cells, and the results might explain the traditional use of Angelica decursiva as remedy for diverse inflammatory pulmonary diseases.
