Effect of melatonin receptor agonist Neu-P11 on phosphorylation of protein kinase B in mouse 3T3-L1 adipocytes
10.3724/SP.J.1008.2015.00802
- Author:
Xiu-Ping LI
1
Author Information
1. Department of Laboratory, Hunan University of Medicine
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Luzindole;
Melatonin;
Neu-P11;
Protein kinase B
- From:
Academic Journal of Second Military Medical University
2015;36(7):802-804
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The objective of present study was to examine the changes of melatonin receptor agonist Neu-P11 on expression of PKB and P-PKB in 3T3-L1 adipocytes and to research the variation mechanisms.Methods: 3T3-L1 pre-adipocytes were cultivated and differentiated in adipogenic cocktail by IBMX, DEX and insulin; Confirm the establishment of insulin resistance model. The changes of PKB and P-PKB protein expressions before and after drug action were detected by Western blot; Results: Expression of PKB and P-PKB reduced significantly by comparison with IS (P < 0.05). Melatonin and Neu-P11 can increase obviously the expression of PKB and P-PKB (P < 0.05). But Luzindole can block the effect of melatoninl, Neu-P11 increasing PKB (P < 0.05), will also be able to block that the melatonin increase P- PKB (P < 0.05), but it can"t block that Neu-P11 increases P - PKB .
