Protective effects of salvianolic acid B on MPP+ -induced mitochondrial injury in PC12 cells
10.3724/SP.J.1008.2015.00685
- Author:
Jun-Ling YANG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
1-methyl-4-phenycpyridiniun;
Mitochondria;
Parkinson disease;
PC12 cells;
Salvianolic acid B
- From:
Academic Journal of Second Military Medical University
2015;36(6):685-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of SalB on 1-methyl-4-phenylpyridinium (MPP+)-induced mitochondrial dysfunction in PC12 cels and the potential mechanism. Methods The injured model of PC12 cels was established by exposing to MPP+ and the mitochondrial function was evaluated by mitochondrial membrane potential (MMP) and mitochondrial ATP synthesis. PCR was used to measure the mitochondrial DNA (mtDNA) content and the expression of PGC- 1, NRF-1 and TFAM. The expression of mitochondrial dynamic related proteins was detected by Western blotting analysis. Results SalB significantly attenuated the MPP+-induced decrease in MMP and mitochondrial ATP synthesis(P<0. 05) , and it significantly increased mtDNA content and the expression of PGC-1, NRF-1 and TFAM mRNA after MPP+ exposure (P< 0. 05). Moreover, Western blotting analysis showed that SalB significantly increased the expression of Opa-1 and Mfn-1 protein, but decreased that of Drp-1 and Fis-1 after MPP+ treatment (P<0. 05). Conclusion SalB can protect PC12 cels against MPP+-induced mitochondrial dysfunction, probably through regulating mitochondrial biogenesis and mitochondrial fusion related proteins.
