Identification of 9,11-secosterols from the south china sea gorgonian subergorgia suberosa
10.3724/SP.J.1008.2015.00655
- Author:
Wen-Qing MIAO
1
Author Information
1. Department of Medicinal Chemistry, School of Pharmacy, Fujian University of Traditional Chinese Medicine
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2015;36(6):655-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigative the chemical components of Subergorgia suberosa collected from the South China Sea. Methods The Et2 O extract of S. Suberosa was purified by repeated column chromatography on silica gel, Sephadex LH- 20 , ODS, and semi-preparative RP-HPLC. The structures of obtained compounds were determined by spectroscopic analysis and comparison with previously reported data. The anti-tumor activity of 10 obtained 9, 11-secosterols were tested with human lung adenocarcinoma A549 cell line and human osteo—sarcoma MG63 cell line the antifungal activities of them were tested with Candida albicans and Aspergillus /umigatus in vitro. Results and Conclusion Ten 9, 11—secosterols were isolated from Subergorgia suberosa collected from the South China Sea. Compounds 2 and 7 have been obtained for the first time from the genus of gorgonian Subergorgia. In vitro anti-tumor experiment showed that compounds 3 6 and 9 had different degrees of tumor inhibitory effects against cell lines A549 and MG63, with the ICs0 values being 6. 31, 30. 42, 8. 33, 9. 57 and 3. 30 0g/ mL for A549 cell line and 30. 83, 21. 32, 9. 19, 28. 01 and 8. 62 0g/mL for MG63 cell line, respectively. The ten steroids showed no anti-fungi activity against Candida albicans or Aspergillus /umigatus in vitro.
