Negative regulation of glucose transport alleviates microvasculature pathological changes of retinopathy in diabetic mice
10.3724/SP.J.1008.2015.00147
- Author:
Ke SHI
1
Author Information
1. Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
Glucose transporter type 1;
Small interfering RNA
- From:
Academic Journal of Second Military Medical University
2015;36(2):147-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of glucose transporter-1 (GLUT1) suppression on microvasculature pathological changes in diabetic mice. Methods Thirty-six 8-week-old C57BL/6 mice were randomly divided into normal control, diabetic control and GLUT1siRNA treatment groups. Diabetic model was established by intraperitoneal injection of streptozotocin. GLUT1siRNA treatment group received intravitreal injection of siRNA-mediated GLUT1, and the other two groups received equal amount of non-specific siRNA. Twenty-one weeks after diabetic induction, immunoblotting was conducted to examine the expression of GLUT1 and two inflammation factors; ICAM-1 and TNF-α. We also calculated the retinal glucose concentration. Leukostasis assay and vascular leakage assay were utilized to compare the microvasculature pathological changes between the two groups. Results The expression of GLUT1 was significantly down-regulated in GLUT1siRNA treatment group compared with the other two groups (P
