Regulatory effects of long non-coding RNA HIF1A-AS1 on ischemic myocardial reperfusion injury in rats
10.3724/SP.J.1008.2015.00131
- Author:
Guan-Xin ZHANG
1
Author Information
1. Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Autophagy;
Cardiac myocytes;
HIF1A-AS1;
Long non-coding RNA;
Myocardial reperfusion injury
- From:
Academic Journal of Second Military Medical University
2015;36(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory effects of long non-coding RNA HIF1A-AS1 on the myocardial ischemia reperfusion (I/R) injury and the related mechanism. Methods Myocardial I/R injury model was established with SD rats, and hypoxia reoxygenation (H/R) model was established with rat cardiac myocytes. si-HIF1A-AS1 was used to inhibit HIF1A-AS1 expression in the cardiac myoctyes. Then the mRNA expression of HIF1A-AS1 was detected by real-time PCR, the growth vitality of cardiac myocytes was investigated by MTT assay, the concentration of lactate dehydrogenase (LDH) in the culture media was detected by ELISA, and the autophagy-associated protein Beclin-1 expression was observed by Western blotting analysis. Results HIF1A-AS1 expression was increased in cardiac muscle of rat I/R model and rat cardiac myocytes of H/R model. Inhibition of HIF1A-AS1 by siRNA protected the cardiomyocytes against H/R injuries, reversing the decreased growth vitality of cardiac myoctyes, increased LDH level in the culture media, and increased expression of autophagy-related protein Beclin-1 induced by H/R stimulation. Conclusion Inhibition of long non coding RNA HIF1A AS1 might play a protective role in I/R injury of cardiac myoctyes by inhibiting the excessive autophagy of cardiomyocytes.
