MiR-141 inhibits the proliferation of head and neck squamous cell carcinoma
10.3724/SP.J.1008.2015.01314
- Author:
Guo-Dong WANG
1
Author Information
1. Department of Stomatology, Changzheng Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Head and neck neoplasms;
MicroRNA-141;
Squamous cell carcinoma;
ZEB1
- From:
Academic Journal of Second Military Medical University
2015;36(12):1314-1318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of miR-141 on the proliferation of head and neck squamous cell carcinoma (HNSCC) cells. Methods The expressions of miR-141 in 19 HNSCC tissues and the matched adjacent normal tissues were analyzed by qRT-PCR. The miR-141 in Hep-2 or SCC-9 cells was overexpressed or down-regulated by miR-141 mimics or miR- 141 ASO transfection. Then the effects of overexpression or down-regulation of miR-141 on cellular proliferation were analyzed by MTT experiment. The targeted genes of miR-141 were predicted by bioinformatics algorithms. Results The expression of miR-141 in HNSCC tissues was found significantly lower than that in matched normal tissue (P<0. 05). Overexpression of miR141 significantly inhibited the proliferation of Hep-2 and SCC-9 cells, and down-regulation of miR-141 significantly promoted the proliferation of Hep-2 and SCC-9 cells (P<0. 05). Bioinformatics algorithms showed that ZEB1 was likely to be a targeted gene of miR-141 in HNSCC. Conclusion miR-141 may inhibit HNSCC cell proliferation via ZEB1.
