A novel impedimetric immunosensor based on AgI mimic enzyme nanomaterial for detecting carcinoembryonic antigen
10.16781/j.0258-879x.2016.12.1533
- Author:
Yan-Ling ZHAO
1
Author Information
1. College of Chemistry and Chemical Engineering, Taiyuan University of Technology
- Publication Type:Journal Article
- Keywords:
Biosensing techniques;
Carcinoembryonic antigen;
Chitosan;
Electric impedance;
Electrochemical immunosensor;
Mimic enzyme;
Nanoparticles;
Silver iodide
- From:
Academic Journal of Second Military Medical University
2016;37(12):1533-1537
- CountryChina
- Language:Chinese
-
Abstract:
Objective Todesign a novel impedimetric immunosensor basedon AgI mimic enzyme nanomaterial for detecting carcinoembryonic antigen (CEA) in serum with high sensitivity. Methods A novil chitosan modified AgI (CS-AgI) nanomaterial was synthesized and was characterized by transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR). Then by utilizing CS-AgI labeled CEA antibody as tags, we prepared a novel impedimetric immunosensor on the gold electrode using the sandwich-type immunoassay. The electrochemical propertiesof the prepared impedimetric immunosensor were observed by electrochemical workstation and the concentration of CEA in sample was quantitatively analyzed. Results The synthesized CS-AgI nanoparticles were spherical in shape, with the particle size being 100 nm; the particles were employed to construct immunosensor as signal markers. The immunosensor had an excellent electrochemical performance in detection of CEA under PBS base solution of pH = 7, and its AC impedance response increased with the increase of the logarithm of CEA concentration, exhibiting a good linear relationship in the range of 0. 1ng/mL to 80ng/mL (r = 0.996), with a detection limkof 0. 05 ng/mL. Conclusion Based on AgI mimic enzyme nanomaterial, the impedance immunosensor prepared in this study shows a high sensitivity in detecting CEA; meanwhile, t has acceptable selectivity, repeatability and stability, providing an experimental evidence for early diagnosis and treatment of cancer.
