Preparation of PCM modified liposome and evaluation of its myocardium targeting efficiency
10.16781/j.0258-879x.2016.11.1348
- Author:
Xin WANG
1
Author Information
1. Chongqing Medical Engineering Research Center, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Cardiac myocytes;
Liposomes;
PCM;
Plasmids;
Transfection efficiency
- From:
Academic Journal of Second Military Medical University
2016;37(11):1348-1352
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare PCM modified liposome (PCM-LIP) containing enhanced green fluorescent protein expression plasmid (pEGFP) and to evaluate its myocardial targeting ability. Methods Liposome was prepared by film-ultrasonic, with PCM used as ligand and DOTAP as cationic lipid material. PCM-LIP containing pEGFP was prepared by incubating liposome with pEGFP at room temperature. The connecting method of PCM was optimized and the connection rate of PCM was determined. The characteristics of liposomes including encapsulation ability, morphology, particle size, zeta potential and stability in phosphate buffer solution (PBS) were observed. The transfection efficiency of liposomes into H9c2 cells was evaluated by inverted fluorescence microscopy and flow cytometry, so as to characterize their myocardial targeting ability and to determine the optimum dosage of PCM. Results PCM-LIP was prepared by insertion method and the amount of PCM accounted for 3% of the lipid. After incubation with pEGFP, PCM-LIP was spherical in shape, with the particle size being (261. 9±2. 2) nm, zeta potential being (-5. 0±0. 6) mV, and PCM-LIP was stable in PBS at -4℃ for 30 d. The transfection efficiency of PCM-LIP was significantly higher than that of unmodified liposome (P<0. 05). Conclusion PCM can improve the transfection efficiency of liposome into cardiomyocytes and PCM-LIP shows a satisfactory myocardial targeting ability.
