Determination of testosterone and cortisol in saliva of healthy human by liquid chromatography-tandem mass spectrometry
10.16781/j.0258-879x.2019.01.0104
- Author:
Wei-Feng GU
1
Author Information
1. Department of Outpatient, Shanghai Naval Vessel Service Department
- Publication Type:Journal Article
- Keywords:
Cortisol;
Endogenous hormones;
Liquid chromatography-tandem mass spectrometry;
Saliva;
Testosterone
- From:
Academic Journal of Second Military Medical University
2019;40(1):104-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a convenient, reliable and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of two kinds of endogenous hormones (testosterone, cortisol) in the saliva of healthy human. Methods A positive ion mode was performed on the Agilent 6410A mass spectrometer. Carbamazepine was used as the internal standard. The saliva samples were centrifuged at 21 912.8×g for 10 min with OMGEA NANOSEP 10K ultrafiltration tubes, and chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column with isocratic elution. The mobile phase was acetonitrile∶0.1% formic acid awter solution (60∶40); flow rate was 0.3 mL/min; injection volume was 10 μL; column temperature was 25 ℃; and the analysis period of each sample was 3 min. Results Testosterone and cortisol had good linear relationship with correlation coefficient (r) being both 0.990. Inter-and intra-day precision was both less than 15%. Conclusion This method can be used to determine the contents of testosterone and cortisol in saliva, which provides a reference for rapid and simple detection of hormone levels in vivo.
