Near-infrared spectroscopy in rapid determination of glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian
10.16781/j.0258-879x.2020.07.0798
- VernacularTitle: 近红外光谱法快速测定甘草饮片中甘草酸和甘草苷的含量
- Author:
Hong-Bin GAO
1
Author Information
1. Department of Pharmacy, Changhai Hospital, Naval Medical University (Second Military Medical University)
- Publication Type:Journal Article
- Keywords:
Glycyrrhizae Radix et Rhizoma;
Glycyrrhizic acid;
Herbal pieces for decoction;
Liquiritin;
Near-infrared spectroscopy;
Rapid determination
- From:
Academic Journal of Second Military Medical University
2020;41(7):921-925
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid determination model based on near-infrared spectroscopy (NIRS) for glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian. Methods The contents of glycyrrhizic acid and liquiritin in Glycyrrhizae Radix et Rhizoma Yinpian from different places of origin were determined by high performance liquid chromatography (HPLC) as reference values. At the same time 2 200-2 049, 1 750-1 450, 1 151-1 001 nm and 1 795-1 475, 1 395-1 293, 1 125-1 030 nm wavelength ranges of near-infrared spectra were selected to establish the rapid determination model by combining partial least squares (PLS) regression analysis with cross validation method. Results The correlation coefficient and root-mean-squares error of cross validation of the established content calibration model were 0.980 and 0.184 for glycyrrhizic acid, and 0.919 and 0.144 for liquiritin, respectively. Conclusion The NIRS-PLS method is convenient, rapid and nondestructive for the content determination of glycyrrhizic acid and liquiritin for large number of Glycyrrhizae Radix et Rhizoma Yinpian, which provides a new and feasible method for the rapid quality evaluation of Glycyrrhizae Radix et Rhizoma Yinpian.
