Low frequency pulsed electromagnetic fields enhance bone morphogenetic protein 9-induced osteogenic differentiation of periodontal ligament stem cells
10.16781/j.0258-879x.2020.07.0737
- VernacularTitle: 低频脉冲电磁场增强骨形态发生蛋白 9 诱导牙周膜干细胞成骨分化作用
- Author:
Yan-En WANG
1
Author Information
1. Department of Stomatology, Changhai Hospital, Naval Medical University (Second Military Medical University)
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic protein 9;
Low frequency pulsed electromagnetic fields;
Osteogenic differentiation;
Periodontal ligament stem cells;
Window effect
- From:
Academic Journal of Second Military Medical University
2020;41(7):855-863
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the low-frequency pulsed electromagnetic fields (LF-PEMFs) can enhance the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) induced by bone morphogenetic protein 9 (BMP9). Methods We isolated CD146＋/STRO-1＋ cells (namely PDLSC) from periodontal ligament cells of healthy human premolars, transfected the PDLSC with BMP9-overexpressing recombinant adenoviruses (Ad-GFP-BMP9), exposed the cells to the different intensities of PEMF stimulation (15 Hz, 0.6，1.2，1.8，2.4，3.0 mT, 1 h/12 h), and then detected the expression of runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) by qRT-PCR and Western blotting. Results Overexpression of BMP9 significantly promoted the expression of osteogenic markers (Runx2, ALP, OCN and OPN) in PDLSC (P0.05). After the intervention with 1.2, 1.8, 2.4 and 3.0 mT PEMF, the expression levels of osteogenic markers in PDLSC were significantly higher than those exposed to BMP9 alone (P0.05), and reached the peak at 2.4 mT (P0.05), indicating that LF-PEMFs enhanced BMP9-induced osteogenic differentiation of PDLSC, and there was a “window effect”. Conclusion LF-PEMFs stimulation (15 Hz, 1.8 to 3.0 mT) can enhance BMP9-induced osteogenesis of hPDLSCs in vitro.
