Analysis of drug - resistant gene polymorphisms in Plasmodium falciparum imported from Equatorial Guinea to Shandong Province in 2015 and 2016
10.16250/j.32.1374.2020114
- VernacularTitle:2015—2016 年山东省由赤道几内亚输入的恶性疟原虫抗药性基因多态性分析
- Author:
Guang-Kui NIE
1
,
2
,
3
;
Chao XU
1
;
Qing-Kuan WEI
1
,
2
;
Jin LI
1
;
Ting XIAO
1
;
Hui SUN
1
,
2
;
Xiang-Li KONG
1
;
Kun YIN
1
,
2
;
Gui-Hua ZHAO
1
;
Ben-Guang ZHANG
1
;
Ge YAN
1
;
Bing-Cheng HUANG
1
,
2
Author Information
1. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, China
2. School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Jinan, China
3. Jining Health School, Shandong Province, China
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
Imported malaria;
Equatorial Guinea;
Drug resistance;
Gene polymorphism
- From:
Chinese Journal of Schistosomiasis Control
2020;32(6):612-617
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the drug-resistant gene polymorphisms in Plasmodium falciparum imported from Equatorial Guinea to Shandong Province. MethodsFrom 2015 to 2016, blood samples were collected from imported P. falciparum malaria patients returning from Equatorial Guinea to Shandong Province, and genome DNA of the malaria parasite was extracted. The drug-resistant Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, and K13 genes of P. falciparum were amplified using a PCR assay, followed by DNA sequencing, and the sequences were aligned. Results The target fragments of all 5 drug-resistant genes of P. falciparum were successfully amplified and sequenced. There were 72.8%, 18.6%, and 8.6% of P. falciparum parasites carrying the wild-, mutant-, and mixed-type Pfcrt gene, respectively, and all mutant haplotypes were CVIET (the underline indicates the mutation site). There were 20.0%, 61.4% and 18.6% of P. falciparum parasites carrying the wild-, mutant-, and mixed-type Pfmdr1 gene, respectively, and the mutant haplotypes mainly included YF and NF (the underlines indicate the mutation sites). There were 1.4%, 98.6%, and 0 of P. falciparum parasites carrying the wild-, mutant-, and mixed-type Pfdhfr gene, respectively, and AIRNI was the predominant mutant haplotype (the underline indicates the mutation site). There were 1.4%, 94.3%, and 4.3% of P. falciparum parasites carrying the wild-, mutant-, and mixed-type Pfdhps gene, respectively, and SGKAA was the predominant mutant haplotype (the underline indicates the mutation site). The complete drug-resistant IRNGE genotype consisted of 8.6% of the Pfdhfr and Pfdhps genes, and the K13 gene A578S mutation occurred in 1.4% of the parasite samples. Conclusions There are mutations in the Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, and K13 genes of P. falciparum imported from Equatorial Guinea to Shandong Province, with a low frequency in the Pfcrt gene mutation and a high frequency in the Pfmdr1, Pfdhfr, and Pfdhps gene mutations, and the K13 gene A578S mutation is detected in the parasite samples.
