The anti-aging effects of different parts of Scutellaria baicalensis Georgi based on 1H NMR urine metabolomics
10.16438/j.0513-4870.2020-0865
- VernacularTitle:基于1H NMR尿液代谢组学的黄芩不同生长部位抗衰老作用研究
- Author:
Meng-ru LI
1
;
Yu-zhi ZHOU
1
;
Jian-xin CHAI
2
;
Qiang CHEN
2
;
Li GAO
1
;
Guan-hua DU
1
,
3
;
Xue-mei QIN
1
Author Information
1. Modern Research Center of Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. Shanxi Zhen Dong Pharmaceutical Co., Ltd., Changzhi 047100, China
3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:Research Article
- Keywords:
urine;
metabolomics;
italic>Scutellaria baicalensis;
aging;
ifferent growth site;
leaf
- From:
Acta Pharmaceutica Sinica
2020;55(11):2702-2712
- CountryChina
- Language:Chinese
-
Abstract:
The effects of alcohol extracts from roots, stems, leaves, and flowers of Scutellaria Baicalensis Georgi (SBG) on endogenous metabolism in D-gal-induced aging-model rats were investigated by 1H NMR metabolomics. Results showed that 32 endogenous metabolites were identified in the urine. Combined with the VIP value and t-test, 14 different metabolites were found by multivariate statistical analysis of the spectrum. Compared with the control group, the content of α-ketoglutaric acid, hippuric acid and 3-hydroxybutyrate in the urine of rats in the model group was significantly decreased (P<0.05) and the content of trimethylamine oxide, glycine, alanine, lactic acid, dimethylglycine, acetate, pyruvate, taurine, allantoin, betaine, N-acetylated glycoprotein was significantly increased (P<0.05). The metabolites were mainly derived from taurine and hypo-taurine metabolism; glycine, serine and threonine metabolism; pyruvate metabolism; glycolysis/gluconeogenesis; glyoxylic acid and dicarboxylic acid metabolism; and the tricarboxylic acid cycle. The content of differential metabolites in urine samples was altered by the alcohol extracts from the different parts of SBG. Leaves extracts of SBG had the greatest effect on urine metabolites, and mainly affected taurine and hypo-taurine metabolism; glycine, serine and threonine metabolism; and pyruvate metabolism. This study provides a reliable experimental basis for the future development of SBG. This animal experiment was approved by the Committee on the Ethics of Animal Experiments of Shanxi University (SXULL2016036).
