Molecular Characterization of a New Hantaan Virus Howang Strain.
- Author:
Yong Kyu CHU
;
Ho Wang LEE
- Publication Type:Original Article
- MeSH:
Base Sequence;
Genome;
Hantaan virus*;
Hemorrhagic Fever with Renal Syndrome;
Hydrophobic and Hydrophilic Interactions;
Nucleotides;
Open Reading Frames;
Peptides;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length
- From:Journal of the Korean Society of Virology
1997;27(1):59-68
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Hantaan virus Howang strain which isolated from the blood of severe case of Korean hemorrhagic fever is more virulent than HTN 76/118 and showed different RFLP from partial PCR amplified M genome segment to established Hantaan serotype viruses. We have determined the nucleotide sequence of the M and S genome segments and compared to HTN 76/118. The M and S segment of Howang strain has 3615 and 1696 nucleotides long, respectively. The M segment sequence of Howang strain is one mucleotide shorter than HTN 76/118. The sequence data of Howang strain shows 93.5% homology to HTN 76/118. One long open reading frame, which stoats from 41nt. to 3448nt. of the M segment and from 37nt. to 1326nt. of the S segment, exist to on complementary sense of the virus genome. There are no significant difference between HTN 76/118 and Howang strain on hydrophobicity of deduced polypeptides, but has slight difference on secondary structure.
