Screening of Anti-HIV-1 Activity of Natural Product by MTT Assay.
- Author:
Joo Shil LEE
;
Jeong Gu NAM
;
Chun KANG
;
Hong Rae LEE
;
Young Jong LEE
;
Yung Oh SHIN
- Publication Type:Original Article
- MeSH:
Biological Products;
Cell Line;
Chloroform;
Chromatography, Gel;
Curcuma;
Lamiaceae;
Lithospermum;
Lonicera;
Magnetic Resonance Spectroscopy;
Magnolia;
Mass Screening*;
Methanol;
Portulaca;
Spectrum Analysis;
Water
- From:Journal of the Korean Society of Virology
1997;27(1):87-95
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Methanol and/or boiling water extraction of 201 natural products and subsequent MTT assay using MT-4 cell line was carried out to screen the anti-HIV-1 activity. Among 97 methanol extracts, 7 extracts from Chrysanthemi Indicium Flos, Magnoliae Cortex Machili Cortex, Reynoutriae Rhizoma, Lithospermi Radix Agastachis Herba, and Chaenomelis Fructus showed anti-HIV-1 activity and their SI value were 2.25 to 5.77. In addition, among 119 boiling water extracts, 10 extracts from Lonicerae Caulis et Foloium, Elsholtziae Herba, Leonuri Herba, Portulacae Herba, Schizonepetae Herba, Curcumae Rhizoma, Amomi Cardamomi Fructus, Cirsii Radix et Herba, Carpesii Herba, and Siegesbeckiae Herba showed anti-HIV-1 activity and their SI value were 1.30 to 7.64. Methanol extracts of above seven natural products were fractionated and the anti-HRs_1 activity of each fraction was examined. Extraction was carried out with hexane, chloroform, butanol, and water to trace active anti-HIV-1 componets. As a result, the water fraction of Magnoliae Cortex, Machili Cortex, Reynoutriae Rhizoma, Agastachis Herba, Chaenomelis Fructus and the butanol fraction of Chrysanthemi Indicium Flos, Reynoutriae Rhizoma showed anti-HIV-1 activity and their SI value were 1.40 to 8.02. We could reach a conclusion that studies to trace the anti-HIV-1 active component of each natural products in further Sractionation and to identify its structure by Infrared spectroscopy, NMR spectroscopy and gel permeation chromatography were needed.
