Heavy Metal Tolerance of Novel Papiliotrema Yeast Isolated from Vietnamese Mangosteen
10.1080/12298093.2020.1767020
- Author:
Kim Cuc Thi NGUYEN
1
;
Phu Van NGUYEN
;
Hai Thi Hong TRUONG
Author Information
1. Institute of Biotechnology, Hue University, Hue, Viet Nam
- Publication Type:RESEARCH ARTICLE
- From:Mycobiology
2020;48(4):296-303
- CountryRepublic of Korea
- Language:English
-
Abstract:
Three yeast strains (Hue-1, Hue-8, and Hue-19) with strong heavy metal tolerance were isolated from mangosteen from Hue city, Vietnam. They exhibited identical phenotype and phylogeny. Sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region demonstrated that the closest relative of these strains is Papiliotrema sp. with 2.12% and 3.55–3.7% divergence in the D1/D2 domain, and ITS domain, respectively. Based on the physiological, biochemical, and molecular data, the three strains belong to a novel species of Papiliotrema genus, for which the namePapiliotrema huenov sp. nov. is proposed. These strains are highly tolerant of heavy metals compared to other yeasts, being able to grow in the presence of 2 mM Pb (II), 2 mM Cd (II), and up to 5 mM Ni (II), but no growth was observed in the presence of 1 mM As (III).
