Cytotoxicity of natural killer cells on canine mammary carcinoma cells
10.14405/kjvr.2020.60.1.25
- Author:
Da-Un JEONG
1
;
Jeong Su BYEON
;
Na-Yeon GU
;
Moonhee JUNG
;
Eun Hee KIM
;
Hyung-Seok KIM
;
In-Soo CHO
;
Jae-Young SONG
;
Bang-Hun HYUN
;
Jienny LEE
Author Information
1. Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
- Publication Type:Original Article
- From:Korean Journal of Veterinary Research
2020;60(1):25-32
- CountryRepublic of Korea
-
Abstract:
Natural killer (NK) cells play have a crucial role in the early phase of immune responses against various pathogens. We compared characteristics of canine NK cells against two canine mammary carcinoma cell lines, REM134 and CF41.Mg. REM134 showed higher expression of progesterone receptor, proliferative cell nuclear antigen, Ki67, multiple drug resistance, Bmi-1, c-myc, E-cadherin, and human epidermal growth factor receptor type-2 than that of CF41.Mg. For specific expansion and activation of NK cells, we isolated CD5 negative cells from canine peripheral blood mononuclear cells and co-cultured K562 cells in the presence of interleukin (IL)-2, IL-15, and IL-21 for 21 days. As a result, we found that expression markers of activated NK cells such as NKp30, NKp44, NKp46, NKG2D, CD244, perforin, granzyme B, and tumor necrosis factor alpha were highly upregulated. In addition, we found there was upregulated production of interferon gamma of activated NK cells against target cells such as REM134 and CF41.Mg.Specifically, we observed that cytotoxicity of NK cells against target cells was more sensitively reacted to CF41.Mg than REM134. Based on the results of this study, we recommend the development of an experimental application of CF41Mg, which has not been reported in canine mammary carcinoma research.
