Polysaccharide from Hizikia Fusiformis Enhances the Immunomodulatory Activity of Macrophages
- Author:
Jieun LEE
1
;
Moon Hee CHOI
;
Eun-su JANG
;
Hyun-Jae SHIN
;
Jung Heon LEE
Author Information
- Publication Type:Original Article
- From:Journal of Rhinology 2020;27(1):8-15
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Background and Objectives:Hizikia fusiformis is widely used in oriental health food in Japan, China, and Korea, and is known for its anti-oxidation properties.Materials and Method: In this study, we investigated the anti-inflammatory and immune-modulatory effects and mechanisms of Hizikia fusiformis (H. fusiformis) extracts in lipopolysaccharide (LPS)-treated RAW 264.7 cells. RAW 264.7 cells were incubated in the presence of different concentrations of the viscozyme component of H. fusiformis (1, 2, 5, and 10 μg/mL), and changes in expression of pro-inflammatory cytokines (GM-CSF, iNOS, VEGF, and COX-2) were evaluated by real-time PCR and immunoblotting. In addition, the associated signaling pathway including phospho (p)-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 was also evaluated.
Results:The viscozyme component of H. fusiformis downregulated the expression of GM-CSF, iNOS, VEGF, and COX-2 mRNA. The augmented NO and ROS production was decreased by administration of H. fusiformis. The signal intensity of p-pNF-κB 65, p-pIkBa, p-p38, and p-p44/42 protein activated by LPS was ameliorated by administration of the viscozyme fraction in RAW 264.7 cells.
Conclusion:These results suggest that H. fusiformis has potential as a therapeutic agent for inflammatory diseases.
