Genetic Diversity of Schistosoma haematobium Eggs Isolated from Human Urine in Sudan.
10.3347/kjp.2015.53.3.271
- Author:
Juan Hua QUAN
1
;
In Wook CHOI
;
Hassan Ahmed Hassan Ahmed ISMAIL
;
Abdoelohab Saed MOHAMED
;
Hoo Gn JEONG
;
Jin Su LEE
;
Sung Tae HONG
;
Tai Soon YONG
;
Guang Ho CHA
;
Young Ha LEE
Author Information
1. Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
- Publication Type:Original Article
- Keywords:
Schistosoma haematobium;
Sudan;
ITS2;
PCR-restriction fragment length polymorphism (PCR-RFLP)
- MeSH:
Adolescent;
Animals;
Base Sequence;
Child;
DNA, Helminth/genetics;
Female;
*Genetic Variation;
Genotype;
Humans;
Male;
Molecular Sequence Data;
Ovum/classification/cytology;
Parasite Egg Count;
Polymorphism, Restriction Fragment Length;
Schistosoma haematobium/*genetics/*isolation & purification/physiology;
Schistosomiasis haematobia/diagnosis/epidemiology/*parasitology/urine;
Students;
Sudan/epidemiology;
Urine/*parasitology
- From:The Korean Journal of Parasitology
2015;53(3):271-277
- CountryRepublic of Korea
- Language:English
-
Abstract:
The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.
