Modulation of collagen gene expression by colchicine.
- Author:
Young Wook RYOO
;
Kyu Suk LEE
;
Joon Young SONG
- Publication Type:Original Article
- Keywords:
Colchicine;
Collagen Gene;
Fibroblast
- MeSH:
Colchicine*;
Collagen Type I;
Collagen*;
Collagenases;
DNA;
Fibroblasts;
Fibronectins;
Gene Expression*;
Gout;
Humans;
Metabolism;
Skin
- From:Korean Journal of Dermatology
1993;31(5):696-701
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recent clinial observations have suggested that colchicine, which is in frequent use in gout can affect the conneciive tissue metabolism in skin and other ti.ues. OBJECTIVE: This study suggest that further development of colchiine might provide a novel means of modulating collagen gen expression in patients with fibrotic disease. METHOD: We examed the effect of colchicine on the expres, on of collagen, fibronectin and collagenase gene by skin filroblast culture hy Nort.hern and dot-blot iybridization. Result : The rate of transcription of genomic DNA corresponding o type I collagen and libvonectin was reduced in colchicine-treated cultures but collagenase was not. reduced. Canclusion : The microti.ikile disruptive agent, colchicine, reduced the expression of type I collagen and fibronectin in a dose-(lependent manner. This st.udy suppor that colchicine is one of the promising antifibrogenic drugs curvently being tested as a treatment, of hun an fibrotic disease.
